Accurate 96 Real-Time PCR System
Accurate 96 is a real-time PCR system for fluorescence-based nucleic acid amplification and quantitative analysis, suitable for molecular biology research, clinical testing, quality and safety testing, and forensic applications. It supports multiplex PCR, amplification analysis and melting-curve workflows.
- 96 Wells
- 6.0°C/sec
- Single-Copy Gene
- 4 / 6 Fluorescence Channels
Features of Accurate 96
- Up to 6 fluorescence detection channels allowing multiplex PCR.
- Effectively reduce multi-color crosstalk and edge effect, no ROX correction required to reduce sample and reagent use
- Innovative scanning method and time-resolved signal separation technology to improve detection sensitivity
- Unique edge temperature compensation technology to minimize “edge effect”
- User-friendly software
- Innovative technology with long-lasting LED light provides reliability results
Applications of Accurate 96
Molecular Biology Research
Real-time PCR amplification and quantitative nucleic-acid analysis.
Multiplex PCR
Multi-target fluorescence detection using up to six channels.
Gene Expression Research
Quantitative PCR workflows for gene-expression studies.
Quality & Safety Testing
PCR-based molecular testing in quality-control and safety-related laboratories.
Clinical Research & Testing
Real-time PCR workflows in applicable clinical laboratory research and testing settings.
Forensic Research
PCR-based DNA analysis workflows for forensic laboratory applications.
FAQ
Specifications of Accurate 96
| Model | Accurate 96 |
| Temperature control system | |
| Sample capacity | 0.1ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
| Reaction volume | 10-50μl |
| Thermal cycle technology | Peltier |
| Max. Heating/Cooling rate | 6.0° C/s |
| Heating temperature range | 4-100°C |
| Temperature accuracy | ± 0.2°C |
| Temperature uniformity | ±0.2°C @60°C，±0.2°C @95°C |
| Temperature gradient setting range | 30-100°C |
| Temperature gradient difference setting range | 1-36°C |
| Detection system | |
| Excitation light source | 5/6 monochrome high efficiency LEDs |
| Detection device | MPPC |
| Detection mode | Time-resolved signal separating technology |
| Excitation/detection wavelength range | 455-650nm/510-715nm |
| Fluorescent channels | 4/6 channels |
| Supported dye | FAM/SYBR Green,VIC/HEX ROX,Cy5,Cy3(only for x6) |
| Sensitivity | Single copy gene |
| Resolution | 1.33 folds copy number difference can be distinguished in single-plex qPCR |
| Dynamic range | 10 orders of magnitude copies |
Accurate96 Operation Notes
Please read the following precautions carefully before using the instrument!
| Power Condition |  | 220 V, 50/60 Hz, Voltage fluctuation <10%, the instrument must be well grounded. Warning: Improper grounding may cause electric shock or damage to the instrument. | |||||
| Data cable |  | Must use the original cable in the package. | |||||
| Lock and Unlock | | Machine need to be unlocked with the M4 hex wrench upon arrival before other operation. The instrument must be locked before being transported over long distances. See the unlock/lock operation video for details. | |||||
| Applicable Consumables | Both 100ul white, clear, the equivalent heights 100μl strip/plate tube are applicable with flat cap or optical sealing film. Warning: Forbidden to use consumables of the following two specifications: 1) tubes or 96-well plates higher than 100μl standard height; 2) convex caps. Manufacturer will not take responsible for the damage caused by wrong tubes. If user has demand to use some special consumables, please check with the distributor/ manufacturer before use. | ||||||
 100ul-White |  100ul-Clear |  200ul |  200 ul-Convex caps | ||||
| Consumables placement | Only the same specification PCR tube can be used in same run and tubes should be correctly placed in the corresponding well position. When use few tubes or few strips, place empty 8strips on both sides of block to balance the hot lid is Must. | ||||||
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| Strips on both sides | Place tubes at corners | No support tube | Not in the hole | Use different kind of Tube | |||
| Computer requirements | | 1. The computer system requires win10 system. 2. Both the “Screen Off Settings” and “Sleep Settings” of the computer must be turned off. | |||||
| Software Installation | | 1. The software needs to be installed on the D drive. 2. Install the USB driver first, and then install the software. | |||||
| Program settings | The program stage is displayed below the program. ‘None’ means that there is only heating /cooling, and no fluorescence signal is collected; Set it as ‘Amplification’ means machine will collects fluorescence signal once per cycle at amplification stage, and “End point” must be set. Set it as‘Melting’ means machine will collects fluorescence throughout the heating process at melting stage. “All point” must be set. Type of the program phase must correspond to the fluorescence collection setting. | ||||||
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| Sample setting | | The fluorescence channels corresponding to different wells position must be set before the experiment. The settings of well position, channel, gene name, and sample name all need to correspond to each other. | |||||
Why Choose Us?
One-Station Shopping
“Multiple suppliers” have always been an issue in the procurement process. Drawell as a one-stop laboratory equipment and scientific instruments supplier, can perfectly solve this problem.
Customer-centric Services
User Training – Training by Drawell skilled engineers about installation, debug tests, technical services, etc. It can happen in our factory in China, or at the site in the customers’ country.
Online Support
To discuss the problem and get it resolved, online chats, real-time video calls, and remote guidance. For the after-sales stage, our online technical guidance is free and ready forever.
100% Quality Guarantee
1-year free warranty includes repairing damaged parts and replacing selected ones (shipping extra). 5% is charged to extend it before it expires.
