PCR

PCR

PCR

Drawell provides standard thermal cyclers, gradient thermal cyclers and real-time quantitative PCR systems for routine amplification, PCR condition optimization, fluorescence-based quantification and multiplex detection. The range covers portable small-batch systems through 96-well laboratory platforms for different throughput and workflow requirements.

Polymerase Chain Reaction (PCR) machine are equipped with precise temperature control, rapid heating and cooling rates, and user-friendly interfaces. They are widely used in various fields, including molecular biology, genetics, forensic science, and medical diagnostics.

PCR Instruments

4 Types of PCR Instruments

PCR instrument is also called gene amplification instrument, generally divided into four types: ordinary basic PCR instrument, gradient PCR instrument, real-time fluorescent quantitative PCR instrument, and in-situ PCR instrument.

Ordinary PCR instrument Refers to a PCR instrument that can only run a specific annealing temperature for a PCR amplification

Gradient PCR instrument means that a series of different annealing temperature conditions (usually 12 temperature gradients) can be set for one-time PCR amplification.

In situ PCR instrument is an intracellular gene amplification instrument used to analyze the location of target DNA in the cell.

Fuorescence quantitative PCR instrument is based on the design of a common PCR instrument that adds a fluorescent signal excitation and acquisition system and a computer analysis and processing system to form an instrument with a fluorescent quantitative PCR function.

Choose by PCR System Type

  • Standard Thermal Cycler

For routine PCR amplification when gradient optimization or real-time fluorescence detection is not required.

  • Gradient Thermal Cycler

For PCR method development and experiments requiring different annealing temperatures or more flexible thermal optimization.

  • Real-Time PCR / qPCR

For fluorescence monitoring, quantitative analysis, multiplex detection, genotyping and melt-curve workflows.

Applications of PCR

The application range of PCR instruments is very wide. Almost all life science fields are involved: food testing, clinical testing, disease control, inspection and quarantine, scientific research laboratories, food safety, cosmetics testing, environmental sanitation, etc.

  1. Basic nucleic acid research;
  2. Sequence analysis;
  3. Evolutionary analysis;
  4. Detect gene expression;
  5. Amplify the specific sequence from the cDNA library;
  6. Study the neighboring genes of known fragments or unknown DNA fragments.
application of PCR Instruments

Check Your Fluorescent Dyes Before Ordering

Fluorescence Dye Compatibility1

PCR vs Real-Time PCR: Which System Do You Need?

RequirementThermal Cycler PCRReal-Time PCR / qPCR
DNA AmplificationYesYes
Real-Time Fluorescence MonitoringNoYes
Quantitative AnalysisNo direct real-time quantificationYes
Multiplex Fluorescence DetectionNoModel-dependent
Gradient OptimizationSelected modelsSelected models
Typical ChoiceTC1000 / TC2000 SeriesGentier / Accurate Series

Choose a thermal cycler for routine amplification or PCR condition optimization. Choose a real-time PCR system when fluorescence monitoring, quantitative analysis or multiplex detection is required.

Order Process of PCR

order process of Automatic melting point meter

Why Choose Us?

  • Model Selection Support: Match the PCR type, throughput and fluorescence channels to the workflow.
  • Configuration Confirmation: Check consumables, fluorescent dyes, software and connectivity before ordering.
  • Technical Documentation: Brochures, manuals and operation guidance available for supported models.
  • Application Support: Assistance with instrument setup, PCR program configuration and routine operation.

FAQ

A thermal cycler performs DNA amplification through programmed temperature cycling. A real-time PCR system also monitors fluorescence during amplification for quantitative and other fluorescence-based analyses.

Choose a gradient model when different temperature conditions need to be evaluated during PCR method development or optimization.

The current range includes 16-, 48- and 96-well configurations, depending on the model.

Choose according to the dyes and number of targets used in your assay. Current real-time PCR configurations range from 2 to 6 channels.

Gentier-Mini is the portable 16-well configuration with a compact 3.2 kg body.

No. Quantitative fluorescence analysis requires a real-time PCR / qPCR system rather than a standard thermal cycler.

No. Consumable compatibility varies by model and should be confirmed before ordering.

Provide the required PCR type, sample throughput, gradient requirement, fluorescence dyes/channels and consumable format.

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