Nano Spectrophotometer DW-K5900F

Models: DW-K5900F

The microvolume Spectrophotometer has become a routine instrument in modern molecular biology laboratories. It is an instrument that conducts quantitative and qualitative analysis of substances using the spectrophotometric method, and is commonly used for nucleic acid and protein quantification, microarray, full-wavelength scanning, and quantitative determination of bacterial concentration, etc. By adding a fluorescence module to the micro spectrophotometer, it is often used for the detection of low-concentration nucleic acids and proteins, greatly expanding the detection range.

Advantages of Nano Spectrophotometer DW-K5900F

  1. Five automatic wavelength conversions, automatically matching the optimal wavelength based on sample concentration, without the need for manual setting;
  2. Long-life scintillation xenon lamp, with a maximum of 109 flashes;
  3. Internal display: 10.1-inch touch display, multi-point capacitive touchscreen;
  4. Has pollutant identification function;
  5. Operating system: LINUX system;
  6. Supports languages: 9 languages including Chinese, English, French, German, Arabic, Spanish, Russian, Japanese, and Korean.
  7. Supports USB data output, can be linked to Ethernet, Bluetooth, and Wi-Fi.

Features of Nano Spectrophotometer DW-K5900F

  1. Output port: It has 2 USB-A interfaces, 1 Type C interface, 1 HDMI interface, and 1 Ethernet port, enabling connection and use with various devices such as mice, keyboards, desktop computers, etc.
  2. It has a one-click export function for the spectral graph of the scanning and detection results.
  3. Fluorescence detection has a customizable standard curve module, allowing for more flexible compatibility with more reagent kits and experimental methods.
  4. Fluorescence detection has a kinetic module, enabling fluorescence kinetic detection and drawing kinetic curves.
  5. Nano Gold mode,Judging Peak,auto search wavelength of different particles
  6. STD Curve have mord Fitting method,satisfy diversified experiments requirement.

Specifications

★Optical pathMicrovolume module:1、0.4、0.1、0.04、0.02mm(Auto ranging)Cuvette module:10、5、2、1mm;
Volume requirementsMicrovolume module: 0.3~2μL;Cuvette module:≥1000μL(10mm Optical Path),Or the liquid height is ≥ 8.5mm
Light sourceXenon lamp(10⁹ flashes)
Detector2048(cmos)linear imaging sensor
Test wavelength rangeMicrovolume module:200~850nm;Cuvette module:220~800 nm
Wavelength accuracy±1nm;
Resolution Spectrum Bandwidth2nm(FWHM at Hg 254nm);
Absorbance Accuracy0.002 Abs;
Photometric Accuracy1%(0.988 Absorbance at 257 nm);
★Abs rangeMicrovolume module:0~900 Abs(=10mm Optical path)Cuvette module:0~16 Abs(at 1mm Optical path)
★Concentration rangeMicrovolume module: 2~45000 ng/µL(dsDNA);0.06~1343 mg/mL(BSA)Cuvette module(10mm):0.2~80 ng/µL(dsDNA);0.006~2.38mg/mL(BSA)
Test time<3s
Test repeatability0.002A (1mm Optical path) or 1% CV
Dimensions of the instrument(L*W*H)mm290*340*220
Weight6.8kg
Operating conditionsTemperature: 10 – 45℃; Humidity: Below 85%
Output24V  2.5A   60W
Auto test and zero adjustment,after padetal arm down
★It has the function of intelligent sample detection. It can identify pollutants and provide information prompts as well as the type of pollutants. The types of pollutants that can be identified include: protein, nucleic acid, phenol, isothiocyanate guanidine, and hydrochloric guanidine. Moreover, it has the function of result correction, which can provide the original concentration of the sample, the corrected concentration, and the type of pollutants. This ensures the accurate concentration of the sample and the quality of the sample, as well as the accuracy and best experimental results of the experiment.
Nucleic acid testing moduledSDNA, ssDNA, RNA, User settings, oligoDNA, oligoRNA, Gene chip module
Protein detection module A280、Polypeptide, protein chip, BCA, Bradford, Lowry, Pierce 660 module
OD600 moduleCell Fluid Module
Custom module UV-Vis,STD,Nano Gold,Dynamics
STD curve fitting Linear,Interpolation,2nd/3rd-order polynomial
UV-Vis Cuvette Absorbance
Fluore dynamics mode,can customzise 5 stages’ delay time,interval time,Number of tests

Fluorescence

Optical sourceLong life Xenon flash lamp(109 flashes);
Excitation wavelength range190nm-1000nm;
Emission wavelength range280nm-850nm;
Excitation filterStandard configuration:355nm、485nm、544nm、646nm;
Shutter filterStandard configuration:460nm、538nm、590nm、678nm;
DetectorPhotodiode (320 – 1100nm)
Sample Channel1
sample volume1-20 μL;
Tube type0.5mL pcr tube
Sample volume of tube≥100 μL
Preheating timeNo need for preheating
Measuring speed3s / tube
LinearR2≥0.995;
Linear range5 order of magnitude
Built in reagent calculator to calculate the required amount of dye and buffer for the working solution
Equipped within fluorescence meter mode,can directly measure the fluorescence intensity of the sample
lon Sphere
Within Fluore STD curve mode,can free choose Ex light source,curve type,STD volume and reagent volume.
Within Fluore dynamics mode,can customzise 5 stages’ delay time,interval time,Number of tests

Cuvette stirring10 speed,time adjustable
PrinterBuilt-in thermal printer
Liquid column monitoringwhich can detect any bubbles or other abnormalities to ensure accuracy
Audit trialOnce enabled,it cannot be disabled.It features hierarchical  permissions,with different user groups having different permissions.It can record and query user operation information,meeting customer requirements for electronic record traceability.