Anaerobic bacteriology is an important part of studying the micro-ecological balance, and more than 99% of the alimentary canal flora are anaerobic Incubator.
The isolation, culture, and screening of anaerobic microorganisms are conducive to discovering more microorganisms with beneficial functions and are conducive to further revealing the characteristics of anaerobic microorganisms and their relationship with host nutrition, immunity, and health.
With the deepening of the understanding of anaerobic Incubators, anaerobic culture has attracted more and more attention from researchers. How to choose an anaerobic incubator with good performance has become a hot topic in related fields. Today, Tianmei introduces you to several key indicators for choosing an anaerobic incubator.
The anaerobic state is stable
(1) Good air tightness, so that the inner cavity of the box is at a stable anaerobic level under the operation and maintenance state;
(2) The design of the operation hole is reasonable, which is convenient for the operator to enter and exit the box with both hands, and also reduces the chance of outside air entering the anaerobic inner cavity, ensuring a stable anaerobic level in the inner cavity of the box when the hands are in and out;
(3) The design of the transfer chamber is reasonable, which not only facilitates the rapid passage of samples into and out of the box but also reduces the chance of outside air entering the anaerobic box, ensuring a stable anaerobic level in the box cavity when the sample is transferred;
⑷High-performance material, the anaerobic incubator with strong anti-aging ability can reduce the risk of air leakage due to the aging of the outer wall, and ensure anaerobic stability under normal conditions.
The cultivation of anaerobic bacteria should be carried out in a stable temperature environment, and the stability of the temperature of the anaerobic incubator directly affects the reliability of the cultivation results.
Humidity is stable
The cultivation of anaerobic bacteria should be carried out in a stable humidity environment. Too high or too low a humidity environment can easily lead to the emergence of atypical biological characteristics of bacteria, which directly affects the judgment of identification results.
In addition, because the anaerobic incubator is a closed cavity, the evaporation of a large amount of medium water can easily lead to the production of condensed water, which on the one hand produces biological contamination, and on the other hand, it is easy to make bacterial colonies disappear, resulting in culture failure.
With anti-pollution ability
Since the anaerobic incubator has the dual functions of operation and cultivation, the disinfection of the inner chamber is crucial to the success or failure of the experiment. Therefore, it is very necessary to support ultraviolet disinfection and strong oxidant disinfection functions.
With biological detoxification function
All bacteria, including anaerobic bacteria, will produce metabolic wastes in the process of growth and reproduction, and a considerable variety of anaerobic bacteria can produce metabolic waste gas. In the closed cavity, the concentration of metabolic waste gas gradually increases, which ultimately inhibits the growth and reproduction of viable bacteria.
Effective removal of metabolic waste gas (biological detoxification) in the inner cavity is an important guarantee to ensure the good growth state of anaerobic bacteria.
The use of inoculation loops or inoculation needles to inoculate, transfer seeds, pick a single colony, and other routine operations of microorganisms require fine movements.