LC-MSMS DW-EXPEC5210 Liquid Chromatograph – Triple Quadrupole Mass Spectrometer

DW-EXPEC5210 Liquid chromatograph – triple quadrupole mass spectrometer, integrating the high separation efficiency of liquid chromatograph and the strong identification capability of mass spectrometer, has sufficient sensitivity and selectivity, good stability and strong anti-interference. Therefore, it is applicable to the qualitative and quantitative analysis on high sensitivity of trace pesticides and veterinary drugs in complex matrix.

Work Environment of LC-MSMS DW-EXPEC5210

  1. Work environment temperature: 18-25 ℃
  2. Humidity in work environment: (20 ~ 60)% RH
  3. Power supply: five sets of single-phase (220 ± 20) V AC, 10A, 50 Hz power supply

Features of LC-MSMS DW-EXPEC5210

Workstation software:

  1. Basic features of software system: Windows XP or above operating system. The software can control the liquid chromatograph and mass spectrometer, with built-in data processing and report editing functions. Besides, the software can realize the function configuration and condition optimization of the instrument automatically, the atomatic quantification, the mass spectrometry data analysis, and the establishment and retrieval of spectral database.
  2. The system has the functions of automatic correction and instrument condition monitoring.
  3. The LC-MS operation software can be installed on the personal computer, and used for the offline processing of sample analysis data and report generation.

Applications of LC-MSMS DW-EXPEC5210

  1. Detection of sulfonamides in pork
  2. Detection of carbamate pesticides in water
  3. Detection of perfluorinated compounds in environmental media
  4. Screening of neonatal genetic metabolic diseases
  5. Determination of vitamin D in serum

Specifications of LC-MSMS DW-EXPEC5210

Configuration and Performance Indicators of Liquid Chromatograph
1. Ultra-high pressure gradient pump
Two solutions in A and B, or C and D, can be selected as the flowing phase of the system by switching the solvent selection valve.
A vacuum degasser is equipped, with each pump degassing separately (A/B).
Flow range1-4,000 µL/min
Maximum pressure≥ 18,850psi
Accuracy of flow rate≤1%
Precision of flow rate≤ 0.075% RSD
2. Automatic sample injector
Three injection modesfull loop injection, partial loop injection and microliter pickup.
Injection repeatabilityFull loop injection <0.3% RSD; partial loop injection <0.3% RSD; microliter pickup <1.0% RSD
Cross contamination<0.05%
Maximum sample capacity384 bits, and 96 bits for standard liquid injection.
3. Column oven
Temperature control rangeRoom temperature +5 ℃ – 90 ℃
Temperature control modepreheating of flowing phase + forced air circulation
Maximum column capacitySix 250mm long chromatographic columns can be installed at the same time.
Requirements for configuration and performance indicators of mass spectrometry system
1. Ion source (spray ion source for standard configuration)
Orthogonal vertical spray design is adopted, so that the system has strong anti-pollution capacity and low background noise.
Ion source gas supply1 loop of nitrogen for atomizing and 2 loops of nitrogen for desolvation. Specifically, the flow rate of nitrogen for atomizing is 0-2 L/min, and that for desolvation is 0-15 L/min. The maximum spray voltage is 6kV. The flow rate, voltage and temperature can be set up and run under the software interface, to ensure the maximum ionization efficiency and resistance to matrix interference.
A special exhaust device is set in the ion source to prevent the backflow of gas in the closed ion source cavity, further to reduce the memory effect and pollution of the ion source, decrease the load of the mechanical pump, prolong the service time of the mechanical pump oil, maintain the test environment and ensure the health of the staff.
2. Vacuum interface and ion transmission system
High temperature blowback gas designNitrogen is used as the blowback gas, with a flow rate of 0-5L/min to further remove the solvent and reduce the introduction of neutral molecules.
Heating design of vacuum interfaceIt can be heated to 110 ℃ at most to improve the anti-pollution capacity.
Vacuum interface maintenancesimple cleaning and maintenance, without vacuum unloading. The whole process of daily maintenance and installation can be easily completed in a few minutes.
Ion transmission system4-stage differential vacuum design is adopted, and multiple quadrupole transmission is used for accurate focusing of ions.
3. Mass analysis system
Mass analyzerTriple quadrupole mass analyzer.
QuadrupoleMade of high-precision pure Mo material, capable of ensuring the best mass axis stability.
Collision cellAxial acceleration design is adopted, capable of effectively eliminating the interference of ion pair and ensuring the high-throughput analysis capability. Nitrogen (with purity ≥ 99.999%) is used as the collision gas and supplied in cylinder.
Resolution0.4-2 amu (adjustable)
Mass stabilitybetter than 0.1amu/24 hours.
Scanning speed≥ 20,000 amu/s
Number of MRM channels100 channels/s
Mass range m/z5 – 1,000amu.
Dynamic range6 orders of magnitude.
SensitivityESI+, MRM mode: 1pg reserpine, injected on the column, with S/N≥100000:1ESI-, MRM mode: 1pg chloramphenicol, injected on the column, with S/N ≥ 100,000:1;
Scanning functionsFull scan, selected ion monitoring (SIM), product ion scan, precursor ion scan, neutral loss scan, multiple reaction monitoring (MRM), positive/negative ion switching scan, etc. 
4. Detector
Multiplier technology, without any positive and negative ion discrimination effect, which has long service life, capable of ensuring the long-term data stability.
Pulse-counting detector, to ensure the data reproducibility of low limit of detection.
Positive/negative polarity switching time≤ 50ms
Vacuum systemcomposed of a mechanical pump and a turbo molecular pump, with a differential pumping system formed between the ion transmission area and mass analysis area, functioning for automatic power-off protection.

Accessory system

  1. Computer system: Brand computer with mainstream configuration, 4GB memory, 1TB hard disk.
  2. AC stabilized power supply: 15KVA, input voltage of 140v-300v, output voltage of 220 V ± 1%.
  3. Laser printer: 6018L, black and white laser printer.
  4. Nitrogen generator: The maximum flow is 24L/min and the maximum pressure is 116psi.
  5. Mechanical pump: Pumping speed: 65m3/h, 220V power supply, 800W.