DW-K2025 High Performance Liquid Chromatography

DW-K2025 is based on the principle of liquid chromatography separation and can quickly and accurately analyze the samples to be analyzed. It has the characteristics of high pressure, high speed, high efficiency and high sensitivity. Widely used in environmental protection, food, biology, pharmaceutical, personal care and other fields.

Features of DW-K2025 HPLC

  1. Reliability

With a reliable design and high-quality components, DW-K2025 passes the reliability test performed by the authority, ensuring the long-term running in optimum condition.

  1. Precision

The precision and accuracy of the results are guaranteed with our unique pumping and sampling technology, high sensitivity detector, and powerful data processing software.

  1. Ease of use

The operation is more convenient and efficient with a variety of user-friendly designs.

  1. Compliance

Fully complies with FDA21 CFR Part 11 with database mode and data traceability.

DW-K2025 hplc (4)

Technical Parameters of DW-K2025 HPLC Accessories

DW-K2025 P1/P2/P4 Pump

Specification of DW-K2025 P1/P2/P4 Pump

Flow rate range0.001 mL/min~10.000 mL/min
Pump typeIsocratic(P1), Binary(P2), Quaternary(P4)
Maximum pressure62 MPa
Plunger rinsingSupported

Features of DW-K2025 P1/P2/P4 Pump

  1. Precision

Excellent repeatability: The precise gradient change and retention time are guaranteed with the reciprocating tandem plunger pump and pressure dynamic suppression algorithm. The repeatability of retention time is less than 0.2%.

  1. Reliability

The reliable design makes service life longer.

  • Material: The cam is integrally processed from high-hardness alloy steel and combined with high-frequency heat treatment technology, which makes the surface of the cam is more wear-resistant (the hardness is above 55HRC), and the service life is longer.
  • Power: The high-power customized motor, NSK bearings, and independent air duct make the power and life even better.
  • Gearing: Self-lubricating and wear-resistant materials are used for the piston drive mechanism. Also, the inner wall rifling design prevents the piston from accidentally locking, making the gearing more reliable.
  • Plunger: The patented suspended floating plunger(Patent Number: ZL 2020 2 1896102.3 ) can adapt to the working conditions automatically, which is convenient for disassembly and prevents the eccentric wear of the sealing ring effectively. With the special sealing structure and the automatic cleaning of the plunger, the pump seal is more reliable.
  1. Ease of use

It equipped with an online automatic rinsing mechanism. When the pump is working, the rinsing pump is automatically turned on and runs periodically, which can effectively prevent the crystallization of buffer salts and the growth of microorganisms, and prolong the service life of the plunger and plunger sealing.

  • Easy to purge: Both the workstation and the instrument panel are equipped with a purging function, making it easier to purge.
  • Intelligent solvent switching to improve efficiency: Equipped with a 4-channel solvent selection valve for binary pump. The solvent can be automatically switched according to the method.
  • Independent 4-channel gradient proportioning valve, easy for maintenance: The gradient proportioning valve of the quaternary pump adopts a 4-channel independent design. The intelligent monitoring and diagnosis can realize real-time monitoring and independent replacement of each channel, the whole design can reduce maintenance costs effectively.

DW-K2025AS Autosampler

Specification of DW-K2025AS Autosampler

Sample capacity  108, 2 mL(96&384 well plates)
Injection range  0-100 μL
Injection mode  Low-loss, microliter carrying, full-loop
Injection time Minimum to 5 s
Carryover   0.003%
Degassing unit    Online degassing(optional)
Temperature control  4-40 ℃(optional)

Features of DW-K2025AS Autosampler

  1. Precision
  • Precise injection volume: The DW-K2025 autosampler adopts a patented integrated constant pressure needle (Patent Number: ZL 2020 2 1159205.1) and a precise syringe pump, which ensures accurate injection volume and excellent linearity, making the results more precise and accurate.
  • Low carryover to make results more accurate: The sampling needle is highly polished for the outer surface and passivated for the inner surface, which can effectively reduce the sample residue. The carryover is much lower when needle washing of the outer and inner surfaces are performed.
  1. Reliability
  • Design of needle: With our integrated constant pressure sampling needle (Patent Number: ZL 2020 2 1159205.1), the air pressure inside and outside the sample bottle can be balanced during sampling; the injection port is set on the sidewall to prevent bottle pad debris from clogging the needle during puncture.
  • Design of mechanical arm: The ball monorail design of the mechanical arm effectively prevents the arm from locking during moving. The operation is more stable when combined with the motor closed-loop control and out-of-step protection algorithm.
  • Design of degassing unit of washing liquid: The built-in autosampler degassing unit can be used for degassing of washing liquid, avoiding interference caused by air bubbles
  • Design of self-compensation injection pump: The maintenance-free self-lubricating guide device and self-compensating sliding screw are used for real-time compensation of the slight wear, andit has excellent motion accuracy and is more durable.
  1. Ease of use
  • Patented preloaded sample injection mode(Patent Number: ZL 2020 2 2977790.2), the injection time can be shortened to 5 s, which greatly improves the efficiency.
  • A variety ofinjection modes combined with sample temperature control technology to satisfy different detection needs such as rare and unstable samples.
  • The dehumidification function is optionally equipped to avoid the generation of condensed water, effectively prevent the sample from being diluted, and ensure accurate results.
  • The maintenance is more convenient and efficient with the in-front injection valve.

DW-K2025CO Column Oven

Specification of DW-K2025CO Column Oven

Operating principlePeltier element and fan-based forced air
Temperature range5℃ above ambient to 85℃ 10℃ below ambient to 85℃

Features of DW-K2025CO Column Oven

  1. Precision

Precise temperature control ensures good separation and repeatability

Using fuzzy PID intelligent temperature control algorithm, ban-based air circulation, and multiple insulation layer design, the column temperature is more accurate, stable, and uniform, the temperature stability is ±0.1 ℃, and the temperature for each analysis are constant and consistent.

  1. Reliability

Triple protection, more safe to use

Real-time liquid leak protection, intelligent monitoring of Peltier and cavity temperature, over-temperature power-off protection, triple safety protection designs, effectively prevents accidental liquid leakage and dangers caused by over-heating.

DW-K2025UVD UV-Vis Detector

Specification of DW-K2025UVD UV-Vis Detector

Light source   Deuterium(transmission type) and Tungsten lamp
Wavelength range190-800 nm
Wavelength accuracy+/- 1 nm
Wavelength precision+/- 0.1 nm
Linearity >2.5 AU
Wavelength calibrationMercury lamp

Features of DW-K2025UVD UV-Vis Detector

  1. Precision

(1) Low LOD

The high-throughput optical path, reference subtraction algorithm, and ultra-precise signal acquisition circuit ensure the detector’s excellent sensitivity.

  • Sample:1.0×10-7 g/mL Naphthalene
  • Column: C18, 4.6×250 mm, 5 μm
  • Mobile phase: 95:5=Methanol: Water
  • Flow rate: 1.0 mL/min
  • Injection Volume: 20 μL
  • Wavelength: 254 nm

(2) Wide linearity

The UVD covers a wide linear range of 2.5AU, making it easier to analyze high concentration samples

  1. Reliability

The patented transmission type deuterium lamp and static light path (Patent Number: ZL 2020 2 0832429.8) allow the light source switching without moving of lamp, which is more reliable.

With the patented design of the optical unit (Patent Number: ZL 2020 2 0832429.8) and the light source  module, the service life is much longer.

  1. Ease of use
  • Full-band spectral scanning: A full-band spectral scanning can be performed to easily find the optimal absorption wavelength of the target analyte.
  • Wavelength time programming: Wavelength can be set for different analytes during different periods, to accomplish the high-sensitivity analysis of complex samples
  • Easy to Maintenance and safety

DW-K2025DAD Diode Array Detector

Specification of DW-K2025DAD Diode Array Detector

Light sourceDeuterium (transmission type) & Tungsten lamp
Wavelength rangeWavelength range
Slit width1 nm, 2 nm, 4 nm, 8 nm
Wavelength accuracy+/- 1 nm
Wavelength precision+/- 0.1 nm
Linearity> 2.0 AU
Wavelength calibrationHolmium glass

Features of DW-K2025DAD Diode Array Detector

  1. Precision

(1) Spectral similarity alignment and peak purity determination are performed for precise qualitative and quantitative analysis

DAD provides full-spectrum information. The compounds can be identified by similarity comparison in the spectral database. Peak purity determination can be performed in the workstation.

(2) High sensitivity—Close to UV Detector

High sensitivity mode can be performed for low concentration samples, the signal-to-noise ratio of DAD can be reached to the same level of UVD.

  • Sample: 1.0*10-7 g/mL naphthalene standard solution
  • Column: C 18, 4.6*250 mn, 5 um
  • Mobile phase: 95% methanol +5% water
  • Flowrate: 1.0 mL/min
  • Injection volume: 20 uL
  • Wavelength: 254 nm

(3) Achieving more accurate inspection data by high-resolution spectra.

When compound detection is performed in high-resolution mode, a finer spectral map can be output for better analysis of complex samples.

  1. Ease of use

Supports 12-channel real-time data collection

Significantly enhance the analysis efficiency by:

  • Up to 12 wavelengths can be set for real-time detection;
  • Real-time analysis at a high sampling rate up to 150 Hz;
  • Full-spectrum detection.

DW-K2025FLD Fluorescence Detector

Specification of DW-K2025FLD Fluorescence Detector

Light source150 W Xe lamp
Wavelength range (Excitation)200 to 850 nm Zero order
Wavelength range (Emission)250 to 900 nm Zero order
Detection sensitivity (Raman peak of water S/N)  S/N ≥ 900 (Baseline method)
Wavelength accuracy±3 nm
Wavelength repeatability±0.5 nm

DW-K2025ELSD Evaporative Light-Scattering Detector

Specification of DW-K2025ELSD Evaporative Light-Scattering Detector

Noise≤0.05 mV(1.0 mL/min 100% Methanol, Gain 1.00) 
Drift≤0.3 mV/30 min(1.0 mL/min 100% Methanol, Gain 1.00)
Min. detection limit≤5.0×10-6 g/mL(500pg)

Workstation of DW-K2025

Features of Workstation

  1. User-friendly

(1) Ease of use

  • The workstation interface is flat designed to quickly complete method development, data acquisition, data processing, report editing and printing, etc..
  • The left side of the interface is the navigation area, on which functional modules can be quickly found, such as project management, spectrogram acquisition, and integral processing.
  • The top of the acquisition interface is a common ribbon that allows you to edit and run methods and sequences without the need for multi-level menus.
  • The right side of the acquisition interface is the status monitoring area, which dynamically displays the real-time status of the instrument.

(2) User-friendly design

  • The workstation has built-in the 2020 edition of the “Chinese Pharmacopoeia” and “Chinese Veterinary Pharmacopoeia” standard method library, which can be directly retrieved and recalled to improve work efficiency
  • The workstation provides intelligent diagnostics and maintenance reminders for the instrument.
  1. Precision
  • Powerful data-processing capability

Up to 25 integration events and 3 quantitative calculation methods (external standard method, internal standard method, and normalization method) for complex data.

  1. Compliance
  • Database storage: With the professional database system, the encryption of original files and security of raw data can be guaranteed.
  • Backup and restore: Backup and restore of data can be easily performed in the workstation, which is convenient for users.
  • Multi-level user system: Supports customized multi-level system for access to data, ensuring the effectiveness of the system.
  • Traceability: The workstation complies with FDA 21 CFR Part 11, with complete records and integrity traceability.

Applications of DW-K2025 HPLC

Application of High-Performance Liquid Chromatography
  1. Pharmaceutical Industry

(1) Analysis of ginsenosides of Rg1, Re, Rb1

Referred to 2020 Chinese Pharmacopoeia

  • Column: C 18 4.6×250 mm, 5 μm
  • Mobile phase: A: ACN B: 0.1% phorsphoric acid/water
  • Temperature: 40℃
  • Injection Volume: 10 μL
  • Flow rate: 1.0 mL/min
  • Wavelength: 203 nm

(2) Analysis of specific spectrum of Lonicera japonica dispensing granules

Referred to YBZ-PFKL-2021073

  • Column: C18 4.6×250 mm, 5 μm
  • Mobile phase: A: ACN B: 0.4% phosphoric acid/water
  • Temperature: 35℃
  • Injection Volume: 15 μL ·Flow rate: 1.0 mL/min
  • Wavelength: 350 nm
  1. Environmental Industry

(1) Analysis of 16 polycyclic aromatic hydrocarbons in water

Referred to HJ 478-2009

  • Column: C 18 4.6×250 mm, 5 μm
  • Mobile phase: A: ACN B: Water
  • Temperature: 30℃
  • Injection Volume: 10 μL
  • Flow rate: 1.2 mL/min
  • Wavelength: time program

(2) Analysis of aldehyde and ketone compounds in waste gas from stationary pollution sources

Referred to HJ 1153-2020

  • Column: C18 4.6×250 mm, 5 μm
  • Mobile phase: A: Methanol B: ACN C: Water
  • Temperature: 35℃
  • Injection Volume: 10 μL
  • Flow rate: 1.0 mL/min
  • Wavelength: time program
  1. Veterinary Drugs
  • Analysis of the veterinary drug Enrofloxacin

Referred to the 2020 Chinese Veterinary Pharmacopoeia

  • Column: C18 column (4.6×250 mm, 5 μm)
  • Mobile phase: 0.025% mol/L aqueous solution of phosphate (pH to 3.3 with triethylamine) + acetonitrile (83+17)
  • Temperature: 25 °C
  • Injection volume: 10 μL
  • Flow rate: 1.0 mL/min
  • Wavelength: 278 nm
  1. Dairy Products

Dairy immunoglobulin IgG analysis

Referred to T/SSFS0002-2021

  • Column: BioCoreTMSEC-300se column (4.6×250 mm, 5 μm)
  • Mobile phase: 20 mmol/L phosphate solution (pH = 7.0)
  • Temperature: 30 °C
  • Injection volume: 10 μL
  • Flow rate: 0.5 mL/min
  • Wavelength: 214 nm
  1. Food

Analysis of sialic acid in bird’s nest and its products

Referred to GB/T30636-2014

  • Column: 300SCX Cation Exchange Column (4.6×250 mm, 5 μm)
  • Mobile phase: acetonitrile + 0.1% aqueous phosphate solution (90 + 10)
  • Column temperature: 30 °C
  • Injection volume: 10 μL
  • Flow rate: 1.0 mL/min
  • Wavelength: 205 nm
  1. Cosmetics

Analysis of p-phenylenediamine, 2, 5-diaminotoluene, and resorcinol in hair dyes

Referred to “Cosmetic Safety Technical Specifications”

  • Column: C 18 column (4.6×250 mm, 5 μm)
  • Mobile phase: Acetonitrile + methanol + 0.02mol/L ammonium acetate buffer (pH 7.5) (2.5 + 2.5 + 95)
  • Temperature: 25 °C
  • Injection volume: 20 μL
  • Flow rate: 1.0 mL/min
  • Wavelength: 280 nm

DW-K2025 HPLC Display