PCR

Introduction of PCR

Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro. The biggest feature of PCR is that it can greatly increase trace amounts of DNA. . The PCR machine based on polymerase is actually a temperature control device, which cycles repeatedly between denaturation temperature (95°C), renaturation temperature (45~55°C), and extension temperature (70~75°C). PCR is one of the most important technologies so far. Its influence is not limited to the field of biological sciences. Almost everyone can feel the changes brought about by PCR. PCR technology has been widely used in paternity testing and criminal investigations.

  • 4 Types of PCR Instruments
  • PCR instrument is also called gene amplification instrument, generally divided into four types: ordinary basic PCR instrument, gradient PCR instrument, real-time fluorescent quantitative PCR instrument, in-situ PCR instrument.
  • Ordinary PCR instrument Refers to a PCR instrument that can only run a specific annealing temperature for a PCR amplification
  • Gradient PCR instrument means that a series of different annealing temperature conditions (usually 12 temperature gradients) can be set for one-time PCR amplification.
  • In situ PCR instrument is an intracellular gene amplification instrument used to analyze the location of target DNA in the cell.
  • Fluorescence quantitative PCR instrument is based on the design of a common PCR instrument that adds a fluorescent signal excitation and acquisition system and a computer analysis and processing system to form an instrument with fluorescent quantitative PCR function.

Application of PCR

The application range of PCR instrument is very wide. Almost all life science fields are involved: food testing, clinical testing, disease control, inspection and quarantine, scientific research laboratories, food safety, cosmetics testing, environmental sanitation, etc.

1.Basic nucleic acid research

2.Sequence analysis

3.Detect gene expression

4.Amplify the specific sequence from the cDNA library

5.Study the neighboring genes of known fragments or unknown DNA fragments

Evolutionary analysis

 

 

 

Order Process of PCR

 

 

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